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thermal shift buffer  (Thermo Fisher)


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    Thermo Fisher thermal shift buffer
    Thermal Shift Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/thermal+shift+buffer/thermal+shift+buffer/pmc12135873-310-22-35
    Average 90 stars, based on 1 article reviews
    thermal shift buffer - by Bioz Stars, 2026-09
    90/100 stars

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    Protein Thermal Shift assay data documenting the β-clamp thermal stabilisation induced by a subset of the yBRET-identified hit compounds and their analogues. ΔTmB (i.e. ΔTm calculated using the Boltzmann method) is reported as average (with SD indicated by error bars) of 4 replicates; black dots represent individual data points. Analogues of hit compounds 11 and 19 are shown as purple and green bars, respectively (see Tables S3 and S4 for details). Reference compound 5 is represented by the blue bar; no significant thermal stabilisation (PTS) effect was detected for hit compound 7 and its analogues.

    Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

    Article Title: Small molecules targeting the eubacterial β-sliding clamp discovered by combined in silico and in vitro screening approaches

    doi: 10.1080/14756366.2024.2440861

    Figure Lengend Snippet: Protein Thermal Shift assay data documenting the β-clamp thermal stabilisation induced by a subset of the yBRET-identified hit compounds and their analogues. ΔTmB (i.e. ΔTm calculated using the Boltzmann method) is reported as average (with SD indicated by error bars) of 4 replicates; black dots represent individual data points. Analogues of hit compounds 11 and 19 are shown as purple and green bars, respectively (see Tables S3 and S4 for details). Reference compound 5 is represented by the blue bar; no significant thermal stabilisation (PTS) effect was detected for hit compound 7 and its analogues.

    Article Snippet: Protein Thermal Shift TM Buffer (ThermoFisher; 5 μL) was added to each well of a RP, LF, SEMI Sk, 96 well plate (0.2 ml) (#AB19700, BIOplastics).

    Techniques: Thermal Shift Assay, Analogues